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Minors in Nebraska will be able to access gender-affirming care, but only after attending 40 hours of therapy that could push back on their identity.
The suit from Planned Parenthood says the law combines two separate subjects in violation of the state's constitution.
Lawmakers overcame a filibuster to pass the measure. Civil rights activists are outraged and may sue.
The legislature overcame a filibuster against the legislation led by Sen. Machaela Cavanaugh.
The regulations were combined into one bill, which opponents say violates the state constitution's requirement for all bills to be single-subject.
Constitutional amendments to protect abortion will be brought to vote in November in 10 states.
The law bans most abortions after 12 weeks and restricts gender-affirming care for trans people under 19.
The legislation, strongly opposed by Sens. Megan Hunt and Machaela Cavanaugh, will likely come to a final vote Thursday.
